mouse polyclonal antibody against alkaline phosphatase  (R&D Systems)

Journal: Scientific reports

Article Title: Responses of primary osteoblasts and osteoclasts from hemizygous β-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D 3 .

doi: 10.1038/s41598-019-50414-7

Figure Lengend Snippet: Figure 2. (A) A representative immunofluorescence photomicrograph shows expression and localization of ALP protein in primary osteoblasts isolated from wild-type (WT) mice (60 × magnification). The primary osteoblasts were plated on coverslip and incubated with primary antibody against ALP (+Anti-ALP), the negative control was incubated with normal goat IgG (−Anti-ALP). The cells were stained for ALP (green) and nuclei (blue). (B) Quantitative ALP-positive cells in primary osteoblasts which represented as percentage of WT control. Numbers in parentheses are numbers of animals.

Article Snippet: Cells were then incubated with 10 μg/mL goat polyclonal primary antibody against ALP (catalog no. AF2910; R&D Systems, MN, USA) at 4 °C overnight.

Techniques: Immunofluorescence, Expressing, Isolation, Incubation, Negative Control, Staining, Control